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Image Search Results
Journal: World Journal of Gastroenterology : WJG
Article Title: Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line
doi: 10.3748/wjg.v10.i23.3522
Figure Lengend Snippet: Confirmation of the successful transfection of HBx into HepG2 cells by RT-PCR. Lane M: markers (the brightest band of the marker presents the 500 bp); lane A: transfected cells; lane B: transfected cells treated with U0126; lane C: the control.
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), lipofectamine 2 000 and G418 were purchased from Invitrogen Inc.
Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Marker
Journal: World Journal of Gastroenterology : WJG
Article Title: Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line
doi: 10.3748/wjg.v10.i23.3522
Figure Lengend Snippet: Apoptosis index of three different groups of cells after treatment with 5-Fu. A: HepG2/pcDNA3 cells; B: HepG2/ pcDNA3-HBx cells; C: U0126 pretreated transfected cells. D: Apoptotic index quantitated by FACS.
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), lipofectamine 2 000 and G418 were purchased from Invitrogen Inc.
Techniques: Transfection
Journal: World Journal of Gastroenterology : WJG
Article Title: Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line
doi: 10.3748/wjg.v10.i23.3522
Figure Lengend Snippet: Protein levels of MDR associated genes in different groups of cells. Lane1: HepG2/pcDNA3 cells; lane2: HepG2/pcDNA3-HBx cells; lane3: transfected cells treated with U0126. The primary antibody for A, B, C, D was β -actin, MDR-1, MRP-1 and LRP respectively.
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), lipofectamine 2 000 and G418 were purchased from Invitrogen Inc.
Techniques: Transfection
Journal: World Journal of Gastroenterology : WJG
Article Title: Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line
doi: 10.3748/wjg.v10.i23.3522
Figure Lengend Snippet: Increased phosphorylation of ERK induced by HBx in hepatoma cells analyzed by Western blot. Lane 1: trans-fected cells after treated with U0126; lane 2: HBx-expressing cells ; lane 3: control cells. A: Specific antibody for phosphory-lated-ERK; B: Specific antibody for total ERK.
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), lipofectamine 2 000 and G418 were purchased from Invitrogen Inc.
Techniques: Western Blot, Expressing
Journal: World Journal of Gastroenterology : WJG
Article Title: Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line
doi: 10.3748/wjg.v10.i23.3522
Figure Lengend Snippet: Changes of MDR related genes after treated with U0126 (mean ± SD)
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), lipofectamine 2 000 and G418 were purchased from Invitrogen Inc.
Techniques:
Journal: World Journal of Gastroenterology : WJG
Article Title: Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line
doi: 10.3748/wjg.v10.i23.3522
Figure Lengend Snippet: Changes of MDR related proteins after treated with U0126 (mean ± SD)
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), lipofectamine 2 000 and G418 were purchased from Invitrogen Inc.
Techniques: